Article
Core domain mutation (S86Y) selectively inactivates polyubiquitin chain synthesis catalyzed by E2-25K.
Biochemistry - 7 Jul 1998
Mastrandrea L D, Kasperek E M, Niles E G, Pickart C M
Abstract excerpt
The mammalian ubiquitin conjugating enzyme known as E2-25K catalyzes the synthesis of polyubiquitin chains linked exclusively through K48-G76 isopeptide bonds. The properties of truncated and chimeric forms of E2-25K suggest that the polyubiquitin chain synthesis activity of this E2 depends on sp...
Topics
- Alkylation
- Amino Acid Substitution
- Animals
- Binding Sites
- Biopolymers
- Catalysis
- Cattle
- Enzyme Activation
- Humans
- Hydrolysis
- Ligases
- Mutagenesis, Site-Directed
- Peptide Fragments
- Phenotype
- Point Mutation
- Polyubiquitin
- Protein Structure, Tertiary
- Rabbits