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Article

A uniform isopeptide-linked multiubiquitin chain is sufficient to target substrate for degradation in ubiquitin-mediated proteolysis.

The Journal of biological chemistry - 25 May 1990

Gregori L, Poosch M S, Cousins G, Chau V

Abstract excerpt

The proteolytic targeting function of ubiquitin was investigated by a combination of site-specific mutagenesis and covalent modification. Lys48 was replaced by a cysteine via mutagenesis of a synthetic ubiquitin gene to generate the mutant Ub-C48. The single cysteine residue in Ub-C48 can be conv...

Topics

Animals
Cloning, Molecular
Escherichia coli
Genetic Variation
Lysine
Oxidation-Reduction
Peptide Hydrolases
Plasmids
Rabbits
Recombinant Fusion Proteins
Reticulocytes
Ubiquitins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

beta-Galactosidase

Source

Identifiers

PMID: 2160452

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2160452-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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A uniform isopeptide-linked multiubiquitin chain is sufficient to target substrate for degradation in ubiquitin-mediated proteolysis.PMID 2160452Read ↗

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