Article
UV light induces IS10 transposition in Escherichia coli.
Genetics - 1 Jul 1998
Eichenbaum Z, Livneh Z
Abstract excerpt
A new mutagenesis assay system based on the phage 434 cI gene carried on a low-copy number plasmid was used to investigate the effect of UV light on intermolecular transposition of IS10. Inactivation of the target gene by IS10 insertion was detected by the expression of the tet gene from the phage 434 PR promoter, followed by Southern blot analysis of plasmids isolated from TetR colonies. UV irradiation of cells...
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