Detection of RB germline mutations using exon-by-exon heteroduplex analysis compared with SSCP.

Article

Detection of RB germline mutations using exon-by-exon heteroduplex analysis compared with SSCP.

Yan ke xue bao = Eye science - 1 Sept 1996

Zhang Q, Minoda K

Abstract excerpt

PURPOSE: To compare heteroduplex analysis with SSCP and to develop a simple and effective method for mutational screening of RB gene. MATERIALS AND METHODS: Leukocyte DNA was prepared from 12 unrelated Japanese patients with hereditary retinoblstoma. PCR combined with simultaneous nonisotopic het...

Topics

DNA, Neoplasm
Exons
Genes, Retinoblastoma
Humans
Mutation
Nucleic Acid Heteroduplexes
Polymorphism, Single-Stranded Conformational
Retinal Neoplasms
Retinoblastoma
Sequence Analysis, DNA

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