Article
Agrobacterium-mediated transformation of seedling-derived maize callus.
Plant cell reports - 1 Apr 2006
Sidorov Vladimir, Gilbertson Larry, Addae Prince, Duncan David
Abstract excerpt
Efficient production of seedling-derived Type I callus was demonstrated for several corn genotypes including commercial inbred lines. Seeds were germinated on MS-based medium containing 10 mg l(-1) picloram and 3 mg l(-1) 6-benzylaminopurine, which induced the development of axillary buds in the area of coleoptilar node. Nodal sections of 7-10-day old seedlings were isolated, split longitudinally, and placed on...
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