Article
Retroviral-mediated marker gene transfer in hematopoiesis-supportive marrow stromal cells.
Journal of hematotherapy - 1 Jun 1998
Bulabois C E, Yerly-Motta V, Mortensen B T, Fixe P, Remy-Martin J P, Hervé P, Tiberghien P, Charbord P
Abstract excerpt
A Moloney-derived retrovirus containing both LacZ and NeoR genes (G1BgSVNa from Genetic Therapy, Inc.), was used to transduce human and murine bone marrow stromal cells. Different kinds of stromal cells that were able to support hematopoiesis were transduced by incubation for 24 h in the presence...
Topics
- Animals
- Bone Marrow Cells
- Cell Cycle
- Escherichia coli
- Flow Cytometry
- Gene Transfer Techniques
- Genetic Markers
- Genetic Vectors
- Hematopoiesis
- Hematopoietic Stem Cells
- Humans
- Kanamycin Kinase
- Mice
- Moloney murine leukemia virus
- Phenotype
- Recombinant Proteins
- Stromal Cells
- Transfection