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Article

A quantitative assay that evaluates the capacity of human stromal cells to support granulomonopoiesis in situ.

Stem cells (Dayton, Ohio) - 1 May 1994

Tamayo E, Charbord P, Li J, Hervé P

Abstract excerpt

We describe an assay that makes possible the observation of granulomonocytic colonies grown on allogeneic stromal layers and the quantification of the stroma-adherent colony-forming cells (CFC). Stromal layers were generated from Stro-1 positive cells isolated from adherent layers of primary long-term marrow cultures using magnetic beads coated with the Stro-1 antibody. The stromal layers consisted mainly of...

Topics

Antibodies, Monoclonal
Antigens, CD
Antigens, CD34
Bone Marrow
Bone Marrow Cells
Cell Adhesion
Cell Separation
Cells, Cultured
Clone Cells
Culture Techniques
Granulocytes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Hematopoiesis

Hematopoietic Stem Cells

Humans

Phenotype

Source

Identifiers

PMID: 7521240
DOI: 10.1002/stem.5530120306

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7521240-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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