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Article

Incorporation of 1,2,4-triazole-3-alanine into a mutant of phage lambda lysozyme containing a single histidine.

Protein engineering - 1 Mar 1998

Soumillion P, Fastrez J

Abstract excerpt

The only histidine residue in the H31N-H137N double mutant of phage lambda lysozyme (lambdaL), at position 48, was biosynthetically replaced by the analogue 1,2,4-triazole-3-alanine (Taz), the basicity of which is 3 pKa units lower. A histidine-auxotrophic strain was grown to stationary phase by histidine limitation in a synthetic medium, then Taz was added on induction to produce a lysozyme with approximately...

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Identifiers

PMID: 9613845
DOI: 10.1093/protein/11.3.213

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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