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Article

Significance of the enzymatic properties of yeast S39A enolase to the catalytic mechanism.

Biochimica et biophysica acta - 2 Apr 1998

Brewer J M, Glover C V, Holland M J, Lebioda L

Abstract excerpt

The S39A mutant of yeast enolase (isozyme 1), prepared by site-directed mutagenesis, has a relative Vmax of 0.01% and an activation constant for Mg2+ ca. 10-fold higher, compared with native enzyme. It is correctly folded. There is little effect of solvent viscosity on activity. We think that the loop Ser36-His43 fails to move to the 'closed' position upon catalytic Mg2+ binding, weakening several electrostatic...

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PMID: 9602170
DOI: 10.1016/s0167-4838(98)00004-1

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