Preparation by site-directed mutagenesis and characterization of the E211Q mutant of yeast enolase 1.

doi:10.1016/0167-4838(95)00049-z

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Article

Preparation by site-directed mutagenesis and characterization of the E211Q mutant of yeast enolase 1.

Biochimica et biophysica acta - 16 Aug 1995

Sangadala V S, Glover C V, Robson R L, Holland M J, Lebioda L, Brewer J M

Abstract excerpt

The published 'charge shuttle' mechanism of enolase (Lebioda, L. and Stec, B. (1991) Biochemistry 30, 2817-2822) assigns Glu-211 the task of orienting a water molecule that serves as the catalytic base which removes the proton from carbon-2 of the substrate. We prepared the E211Q mutant of yeast...

Topics

Amino Acid Sequence
Base Sequence
Kinetics
Ligands
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation
Phosphopyruvate Hydratase
Saccharomyces cerevisiae

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