Article
The H159A mutant of yeast enolase 1 has significant activity.
Biochemical and biophysical research communications - 5 Oct 2000
Brewer J M, Holland M J, Lebioda L
Abstract excerpt
The function of His159 in the enolase mechanism is disputed. Recently, Vinarov and Nowak (Biochemistry (1999) 38, 12138-12149) prepared the H159A mutant of yeast enolase 1 and expressed this in Escherichia coli. They reported minimal (ca. 0.01% of the native value) activity, though the protein ap...
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