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Article

The H159A mutant of yeast enolase 1 has significant activity.

Biochemical and biophysical research communications - 5 Oct 2000

Brewer J M, Holland M J, Lebioda L

Abstract excerpt

The function of His159 in the enolase mechanism is disputed. Recently, Vinarov and Nowak (Biochemistry (1999) 38, 12138-12149) prepared the H159A mutant of yeast enolase 1 and expressed this in Escherichia coli. They reported minimal (ca. 0.01% of the native value) activity, though the protein appeared to be correctly folded, according to its CD spectrum, tryptophan fluorescence, and binding of metal ion and...

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Identifiers

PMID: 11027610
DOI: 10.1006/bbrc.2000.3618

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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