In-situ competition between protamine and fluorochromes for sperm DNA

doi:10.1093/molehr/4.2.127

Article

In-situ competition between protamine and fluorochromes for sperm DNA

1 Feb 1998

Abstract excerpt

In this study we investigated the relationship between the presence of bound protamine on mouse and human sperm DNA and the level of chromomycin A3 (CMA3) and 4'6-diamidino-2-phenylindole (DAPI) fluorescence. This was accomplished by performing a competition assay between salmon protamine and fluorochromes on decondensed spermatozoa that had their nuclear proteins extracted and were fixed on slides. Various...

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