Article
A panel set for epitope analysis of myeloperoxidase (MPO)-specific antineutrophil cytoplasmic antibody MPO-ANCA using recombinant hexamer histidine-tagged MPO deletion mutants.
Journal of clinical immunology - 1 Mar 1998
Tomizawa K, Mine E, Fujii A, Ohashi Y Y, Yamagoe S, Hashimoto Y, Ishida-Okawara A, Ito M, Tanokura M, Yamamoto T, Arimura Y, Nagasawa T, Mizuno S, Suzuki K
Abstract excerpt
A major target protein of antineutrophil cytoplasmic antibody with a perinuclear staining pattern (P-ANCA) has been identified as myeloperoxidase (MPO). Recombinant deletion mutants of MPO, eight fragments of the heavy-chain subunit, and two fragments of the light chain subunit were expressed in E. coli using a pQE expression vector. The recombinant hexamer histidine-tagged fragments were partially purified as...
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