Article
Development of an assay for bioactive insulin.
Analytical biochemistry - 15 Mar 1998
Okada Y, Yokono K, Katsuta A, Yoshida M, Morita S, Irino H, Goto T, Baba S, Roth R A, Shii K
Abstract excerpt
A method to detect the biological activity of serum insulin has been developed. This method, called a bioactive insulin assay, determines the ability of serum insulin to stimulate the autophosphorylation of insulin receptors in an intact cell system. For this, intact Chinese hamster ovary cells which overexpress the human insulin receptor are treated with serum and then lysed. Autophosphorylation of the insulin...
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