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Article

Horseradish peroxidase: partial rescue of the His-42 --> Ala mutant by a concurrent Asn-70 --> Asp mutation.

Archives of biochemistry and biophysics - 15 Mar 1998

Savenkova M I, Ortiz de Montellano P R

Abstract excerpt

In horseradish peroxidase (HRP), hydrogen bonding of Asn-70 to His-42 enhances the catalytic activity of the histidine, and mutation of His-42 to a neutral residue greatly decreases peroxidase activity. The N70D/H42A HRP mutant is compared here to the previously characterized H42A mutant to determine if the Asp-70 substitution can rescue the catalytic activity. The N70D/H42A and H42A mutants give Compound I...

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Identifiers

PMID: 9514658
DOI: 10.1006/abbi.1997.0559

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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