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Article

Rapid sizing of short tandem repeat alleles using capillary array electrophoresis and energy-transfer fluorescent primers.

Analytical chemistry - 1 Apr 1995

Wang Y, Ju J, Carpenter B A, Atherton J M, Sensabaugh G F, Mathies R A

Abstract excerpt

Genetic typing of the short tandem repeat (STR) polymorphism HUMTHO1 has been performed using capillary array electrophoresis and energy-transfer fluorescent dye-labeled polymerase chain reaction primers. Target alleles were amplified by use of primers labeled with one fluorescein at the 5' end and another fluorescein at the position of the 15th (modified) base to produce fragments that fluoresce in the green...

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PMID: 7537476
DOI: 10.1021/ac00103a010

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