Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Compromised optimal hybridization temperatures may explain PM plus DQA1 anomalies.

Biochemistry and molecular biology international - 1 Jan 1998

Riley D E

Abstract excerpt

The multiplex DNA typing test kit, PM plus DQA1 relies on 36 different oligonucleotides that all must hybridize correctly for accurate DNA typing. The G + C contents of the probe oligonucleotides range from 47% to 83%. Since G + C content directly affects optimal hybridization temperatures, a wide range of optimal hybridization temperatures is expected. This was verified by two empirically tested formulas. The...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to Base Composition
Share to DNA

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Histocompatibility Testing

Share to HLA-DQ Antigens

Share to Nucleic Acid Hybridization

Share to Polymerase Chain Reaction

Share to Temperature

Source

Identifiers

PMID: 9503148
DOI: 10.1080/15216549800201062

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 9 closest

Compromised optimal hybridization temperatures may explain PM plus DQA1 anomalies.PMID 9503148Read ↗

Retrofitting massively parallel sequencing (MPS) for HLA-DQA1 and polymarker (PM) in forensic casework.Semantic proximity Read ↗

Technical aspects of typing for HLA-DP alleles using allele-specific DNA in vitro amplification and sequence-specific oligonucleotide probes. Detection of single base mismatches.Semantic proximity Read ↗

Confirmation of PM typing protocols for consistent and reliable results.Semantic proximity Read ↗

Effect of unexpected sequence interruptions to conventional PCR and repeat primed PCR in myotonic dystrophy type 1 testing.Semantic proximity Read ↗

Enhanced PCR amplification of VNTR locus D1S80 using peptide nucleic acid (PNA).Semantic proximity Read ↗

Simplified PCR-SSO procedure for DQA and DQB typing. Simultaneous hybridization of two probes to one membrane.Semantic proximity Read ↗

Preferential PCR amplification of alleles: mechanisms and solutions.Semantic proximity Read ↗

Chemiluminescent detection of genetic polymorphisms based on mismatch hybridization: application to cytochrome P4501A1.Semantic proximity Read ↗

Enhanced discrimination of single nucleotide polymorphisms by artificial mismatch hybridization.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.