Article
Compromised optimal hybridization temperatures may explain PM plus DQA1 anomalies.
Biochemistry and molecular biology international - 1 Jan 1998
Riley D E
Abstract excerpt
The multiplex DNA typing test kit, PM plus DQA1 relies on 36 different oligonucleotides that all must hybridize correctly for accurate DNA typing. The G + C contents of the probe oligonucleotides range from 47% to 83%. Since G + C content directly affects optimal hybridization temperatures, a wid...
Topics
- Alleles
- Base Composition
- DNA
- HLA-DQ Antigens
- HLA-DQ alpha-Chains
- Histocompatibility Testing
- Humans
- Major Histocompatibility Complex
- Nucleic Acid Hybridization
- Osmolar Concentration
- Polymerase Chain Reaction
- Reagent Kits, Diagnostic
- Temperature