Article
Chemiluminescent detection of genetic polymorphisms based on mismatch hybridization: application to cytochrome P4501A1.
Molecular and cellular probes - 1 Feb 2004
Wu Xiaoming, Zhou Yikai, Xu Shunqing
Abstract excerpt
An assay that makes use of differences in thermal stability between perfectly and imperfectly matched hybrids in combination with a sensitive chemiluminescence detection system was developed and applied to the identification of CYP1A1 polymorphisms. In this assay, two oligonucleotide probes for each polymorphic site were designed: one perfectly matching the wild type allele, the other perfectly matching the...
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