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Article

Comparison of serological and molecular typing for HLA-A and -B on cord blood lymphocytes.

Tissue antigens - 1 Jan 1998

Poli F, Scalamogna M, Crespiatico L, Macchi B, Mistò R, Nocco A, Rossini G, Scarpino C, Sioli V, Sirchia G

Abstract excerpt

HLA class I typing by standard microcytotoxicity testing has been unsatisfactory for 14.5% of 1644 cord blood samples. In this study, we evaluated the capacity of PCR-SSP in solving problems in HLA-A,B typing with serological methods. With this aim we have compared serology with PCR-SSP in 100 cord blood samples with doubtful or unreliable HLA-A,B typing. PCR-SSP was successful in amplifying HLA-A,B alleles in...

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Source

Identifiers

PMID: 9459505
DOI: 10.1111/j.1399-0039.1998.tb02948.x

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