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Article

Isolation of a cDNA Encoding a Novel Member of the Transglutaminase Gene Family from Human Keratinocytes

1 Feb 1998

Abstract excerpt

We developed a method using a single set of degenerate oligonucleotide primers for amplification of the conserved active site of transglutaminases by reverse transcription-polymerase chain reaction (RT-PCR) and identification of the PCR products by cleavage with diagnostic restriction enzymes. We demonstrate amplification of tissue transglutaminase (TGC), keratinocyte transglutaminase (TGK), prostate...

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Identifiers

PMID: 9452468
DOI: 10.1074/jbc.273.6.3452

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