Article
A truncated SV40 large T antigen lacking the p53 binding domain overcomes p53-induced growth arrest and immortalizes primary mesencephalic cells.
Cell and tissue research - 1 Feb 1998
Truckenmiller M E, Tornatore C, Wright R D, Dillon-Carter O, Meiners S, Geller H M, Freed W J
Abstract excerpt
As an alternative to primary fetal tissue, immortalized central nervous system (CNS)-derived cell lines are useful for in vitro CNS model systems and for gene manipulation with potential clinical use in neural transplantation. However, obtaining immortalized cells with a desired phenotype is unpr...
Topics
- Animals
- Antigens, Polyomavirus Transforming
- Binding Sites
- Cell Cycle
- Cell Line, Transformed
- Cell Transformation, Viral
- Fibroblasts
- Mesencephalon
- Neurons
- Optic Nerve
- Phenotype
- Protein Binding
- Rats
- Rats, Sprague-Dawley
- Transfection
- Tumor Suppressor Protein p53