Article
Analysis of V(H) genes rearranged by individual B cells in dermal infiltrates of patients with mycosis fungoides.
Clinical and experimental immunology - 1 Dec 1997
Förste N, Gellrich S, Golembowski S, Rutz S, Audring H, Sterry W, Jahn S
Abstract excerpt
In patients with cutaneous T cell lymphomas such as mycosis fungoides B cells can frequently be detected in the lymphocytic dermal infiltrate. To analyse their immunoglobulin heavy chain gene repertoire, single B cells were obtained from tissue sections of two typical patients with mycosis fungoides using hydraulic micromanipulation followed by specific amplification of the respective gene segments by single-cell...
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