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trans-Complementation of yellow fever virus NS1 reveals a role in early RNA replication

1 Dec 1997

Abstract excerpt

Mutational analysis of the nonstructural protein 1 (NS1) of yellow fever virus (YF) has implicated it in viral RNA replication. To further explore this observation, we sought a method for uncoupling NS1 function from NS1 expression and processing as part of the large YF polyprotein. Here we describe a strategy for providing NS1 in trans, utilizing a noncytopathic Sindbis virus vector. Replication of a defective...

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Identifiers

PMID: 9371625
DOI: 10.1128/jvi.71.12.9608-9617.1997

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