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Article

Trans-complementation of chikungunya virus replicase mutants reveals alphavirus replication complexity and supports antiviral tool development.

PLoS pathogens - 1 Dec 2025

Wang Sainan, Naumenko Krystyna, Mirzajani Sarvandani Mandana, Lello Laura Sandra, Makhotina Anna, Claesson Hanna Carolina, Žusinaite Eva, Merits Andres

Abstract excerpt

Chikungunya virus (CHIKV) is a reemerging alphavirus responsible for large-scale outbreaks in tropical regions. Its RNA replication depends on the assembly of functional replication complexes using the P123 and P1234 polyprotein precursors and their cleavage products, the nonstructural proteins (nsP1-nsP4). To dissect this process, we developed a trans-complementation assay using either plasmid-based expression...

Topics

Chikungunya virus
Virus Replication
Animals
Viral Nonstructural Proteins
Mutation
Humans
Chikungunya Fever
RNA-Dependent RNA Polymerase
Cell Line
Viral Replicase Complex Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Chlorocebus aethiops

Antiviral Agents

Alphavirus

Genetic Complementation Test

Source

Open in PubMed Open full text

Identifiers

PMID: 41452936
DOI: 10.1371/journal.ppat.1013838
PMCID: PMC12768418

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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