Article
HLA-DR and DQ typing by polymerase chain reaction using sequence-specific primer mixes reduces the incidence of phenotypic homozygosity (blanks) over serology.
Transplantation - 27 Dec 1996
Bryan C F, Harrell K M, Nelson P W, Pierce G E, Ross G, Shield C F, Warady B A, Aeder M I, Helling T S, Landreneau M D, Luger A M
Abstract excerpt
Because of the inherent difficulties in allele assignment with HLA-DR serological typing, in 1993 our organ procurement organization-based HLA laboratory replaced serology with the molecular method of polymerase chain reaction using sequence-specific primer mixes (PCR-SSP) to type for DR and DQ at a resolution level equivalent to that of serologically defined antigens. In this study, we compared the incidence of...
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