Article
Functional and structural features of the holin HOL protein of the Lactobacillus plantarum phage phi gle: analysis in Escherichia coli system.
Gene - 15 Sept 1997
Oki M, Kakikawa M, Nakamura S, Yamamura E T, Watanabe K, Sasamoto M, Taketo A, Kodaira K
Abstract excerpt
Lactobacillus plantarum phage phi gle has two consecutive cell lysis genes hol-lys (Oki et al., 1996b). In the present study, functional and structural properties of the hol protein (Hol) were characterized in Escherichia coli. Electron microscopic examinations showed that hol under plac in E. coli XL1-Blue injured the inner membrane to yield empty ghost cells with the bulk of the cell wall undisturbed. Northern...
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