Oligohistidine tag mutagenesis of the lambda holin gene.

doi:10.1128/JB.180.16.4199-4211.1998

Back to search

Article

Oligohistidine tag mutagenesis of the lambda holin gene.

Journal of bacteriology - 1 Aug 1998

Smith D L, Young R

Abstract excerpt

Holins are a diverse group of small integral membrane proteins elaborated by bacteriophages to lyse bacterial hosts and effect release of progeny phages in a precisely timed manner. Recently, the holin S gene of phage lambda was overexpressed and the holin protein was purified to homogeneity by m...

Topics

Alleles
Amino Acid Sequence
Bacteriophage lambda
Base Sequence
Codon
Escherichia coli
Histidine
Membrane Proteins
Molecular Sequence Data
Mutagenesis, Insertional
Oligonucleotides
Viral Proteins

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.