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Article

Oligohistidine tag mutagenesis of the lambda holin gene.

Journal of bacteriology - 1 Aug 1998

Smith D L, Young R

Abstract excerpt

Holins are a diverse group of small integral membrane proteins elaborated by bacteriophages to lyse bacterial hosts and effect release of progeny phages in a precisely timed manner. Recently, the holin S gene of phage lambda was overexpressed and the holin protein was purified to homogeneity by means of an oligohistidine tag procedure and immobilized metal affinity chromatography (D. L. Smith, D. K. Struck, J. M....

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Identifiers

PMID: 9696770
DOI: 10.1128/JB.180.16.4199-4211.1998
PMCID: PMC107418

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Source receipt: pubmed, retrieved 7 Sept 2026.

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