Article
An approach to identify functional homologues and suppressors of genes in fission yeast.
Current genetics - 1 Jul 1997
Grallert B, Nurse P
Abstract excerpt
We have developed a procedure using a bank of temperature-sensitive (ts) mutants of fission yeast to identify mutants which can be rescued by expression of a plasmid-borne gene of interest. The procedure has been used to identify new ts alleles of cdc2 and swi7/poll, a ts mutant rescued by actin, and to identify a ts allele of cdc11 which can be rescued by combined mammalian Myc and Max expression. The procedure...
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