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Article

Cyclophilin active site mutants have native prolyl isomerase activity with a protein substrate.

FEBS letters - 1 Sept 1997

Scholz C, Schindler T, Dolinski K, Heitman J, Schmid F X

Abstract excerpt

The prolyl isomerase activity of cyclophilins is traditionally measured by an assay in which prolyl cis/trans isomerization in a chromogenic tetrapeptide is coupled with its isomer-specific cleavage by chymotrypsin. Two variants of mitochondrial cyclophilin with substitutions in the presumed active site (R73A and H144Q) are inactive in the protease-coupled assay, but show almost wild-type activity in an assay...

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PMID: 9305734
DOI: 10.1016/s0014-5793(97)00979-4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Cyclophilin active site mutants have native prolyl isomerase activity with a protein substrate.PMID 9305734Read ↗

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