Cyclophilin active site mutants have native prolyl isomerase activity with a protein substrate.

doi:10.1016/s0014-5793(97)00979-4

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Article

Cyclophilin active site mutants have native prolyl isomerase activity with a protein substrate.

FEBS letters - 1 Sept 1997

Scholz C, Schindler T, Dolinski K, Heitman J, Schmid F X

Abstract excerpt

The prolyl isomerase activity of cyclophilins is traditionally measured by an assay in which prolyl cis/trans isomerization in a chromogenic tetrapeptide is coupled with its isomer-specific cleavage by chymotrypsin. Two variants of mitochondrial cyclophilin with substitutions in the presumed acti...

Topics

Amino Acid Isomerases
Binding Sites
Carrier Proteins
Chymotrypsin
Endopeptidases
Fungal Proteins
Kinetics
Mitochondria
Mutation
Peptides
Peptidylprolyl Isomerase
Protein Folding
Proteins
Saccharomyces cerevisiae
Spectrophotometry

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