Article
Biochemical Features of Recombinant Human Cyclophilin J.
Anticancer research - 1 Mar 2016
Chen Jian, Liefke Robert, Jiang Lixin, Wang Jiahui, Huang Chaoqun, Gong Zhaohua, Schiene-Fischer Cordelia, Yu Long
Abstract excerpt
AIM: To characterize the biochemical features of the newest member of cyclophilin family of peptidyl-prolyl cis/trans-isomerases (PPIases), cyclophilin J (CYPJ). MATERIALS AND METHODS: PPIase assays were performed on purified hCYPJ and its mutated variants. The substrate specificity, half-maximal inhibitory concentration (IC50) of cyclosporin A (CsA) inhibition and circular dichroism (CD) spectrum of CYPJ were...
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