Article
Isolation and molecular characterization of dTnp1, a mobile and defective transposable element of Nicotiana plumbaginifolia.
Molecular & general genetics : MGG - 1 Jan 1994
Meyer C, Pouteau S, Rouzé P, Caboche M
Abstract excerpt
By Northern blot analysis of nitrate reductase-deficient mutants of Nicotiana plumbaginifolia, we identified a mutant (mutant D65), obtained after gamma-ray irradiation of protoplasts, which contained an insertion sequence in the nitrate reductase (NR) mRNA. This insertion sequence was localized by polymerase chain reaction (PCR) in the first exon of NR and was also shown to be present in the NR gene. The mutant...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- DNA Transposable Elements
- DNA, Complementary
- Genes, Plant
- Molecular Sequence Data
- Mutation
- Nitrate Reductase
- Nitrate Reductases