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Article

Leaderless polypeptides efficiently extracted from whole cells by osmotic shock.

Journal of bacteriology - 1 Sept 1997

Thorstenson Y R, Zhang Y, Olson P S, Mascarenhas D

Abstract excerpt

Three molecular foldases, DsbA, DsbC, and rotamase (ppiA), exhibited the unusual property of accumulating in an osmotically sensitive cellular compartment of Escherichia coli when their signal sequences were precisely removed by mutation. A mammalian protein, interleukin-1 (IL-1) receptor antagonist, behaved in a similar fashion in E. coli when its native signal sequence was deleted. These leaderless mutants (but...

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Identifiers

PMID: 9286985
DOI: 10.1128/jb.179.17.5333-5339.1997
PMCID: PMC179401

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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