Article
Leaderless polypeptides efficiently extracted from whole cells by osmotic shock.
Journal of bacteriology - 1 Sept 1997
Thorstenson Y R, Zhang Y, Olson P S, Mascarenhas D
Abstract excerpt
Three molecular foldases, DsbA, DsbC, and rotamase (ppiA), exhibited the unusual property of accumulating in an osmotically sensitive cellular compartment of Escherichia coli when their signal sequences were precisely removed by mutation. A mammalian protein, interleukin-1 (IL-1) receptor antagon...
Topics
- Amino Acid Isomerases
- Amino Acid Sequence
- Bacterial Proteins
- Carrier Proteins
- Cell Fractionation
- Cell Membrane
- Cell Wall
- Cytoplasm
- Escherichia coli
- Humans
- Interleukin 1 Receptor Antagonist Protein
- Isomerases
- Molecular Sequence Data
- Mutation
- Osmotic Pressure
- Peptidylprolyl Isomerase
- Protein Disulfide-Isomerases
- Protein Folding