Article
Screening for imprinted genes by allelic message display: identification of a paternally expressed gene impact on mouse chromosome 18.
Proceedings of the National Academy of Sciences of the United States of America - 19 Aug 1997
Hagiwara Y, Hirai M, Nishiyama K, Kanazawa I, Ueda T, Sakaki Y, Ito T
Abstract excerpt
A systematic screen termed the allelic message display (AMD) was developed for the hunting of imprinted genes. In AMD, differential display PCR is adopted to image allelic expression status of multiple polymorphic transcripts in two parental mouse strains, reciprocal F1 hybrids and pooled backcross progenies. From the displayed patterns, paternally and maternally expressed transcripts can be unequivocally...
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