Single-step kinetics of HIV-1 reverse transcriptase mutants responsible for virus resistance to nucleoside inhibitors zidovudine and 3-TC.

doi:10.1021/bi970512z

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Article

Single-step kinetics of HIV-1 reverse transcriptase mutants responsible for virus resistance to nucleoside inhibitors zidovudine and 3-TC.

Biochemistry - 19 Aug 1997

Krebs R, Immendörfer U, Thrall S H, Wöhrl B M, Goody R S

Abstract excerpt

Two mutants of HIV-1 reverse transcriptase (RT) associated with high-level resistance of the virus to AZT (RT-AZT: D67N, K70R, T215Y, K219Q, and M41L) or 3-TC (RT-3TC: M184V) were expressed in Escherichia coli and purified. None of these mutants showed significant changes in the affinity and kine...

Topics

Anti-HIV Agents
Drug Resistance, Microbial
HIV Reverse Transcriptase
HIV-1
Kinetics
Lamivudine
Mutation
Reverse Transcriptase Inhibitors
Zidovudine

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