Article
Microtubules orient the mitotic spindle in yeast through dynein-dependent interactions with the cell cortex.
The Journal of cell biology - 11 Aug 1997
Carminati J L, Stearns T
Abstract excerpt
Proper orientation of the mitotic spindle is critical for successful cell division in budding yeast. To investigate the mechanism of spindle orientation, we used a green fluorescent protein (GFP)-tubulin fusion protein to observe microtubules in living yeast cells. GFP-tubulin is incorporated into microtubules, allowing visualization of both cytoplasmic and spindle microtubules, and does not interfere with normal...
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