Article
Quantification of vitamin D receptor mRNA by competitive polymerase chain reaction in PBMC: lack of correspondence with common allelic variants.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research - 1 May 1997
Mocharla H, Butch A W, Pappas A A, Flick J T, Weinstein R S, De Togni P, Jilka R L, Roberson P K, Parfitt A M, Manolagas S C
Abstract excerpt
It has been recently claimed that polymorphism for the vitamin D receptor (VDR) influences several aspects of calcium and bone metabolism. To evaluate the physiologic plausibility of these claims, we compared the abundance of the VDR mRNA in peripheral blood mononuclear cells (PBMCs) between different VDR genotypes using a quantitative reverse transcribed polymerase chain reaction-based method. The method is...
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