Article
Vitamin D receptor mRNA measured in leukocytes with the TaqMan fluorogenic detection system: effect of calcitriol administration.
Clinical chemistry - 1 Aug 2004
Soldati Laura, Adamo Donatella, Bianchin Cristiana, Arcidiacono Teresa, Terranegra Annalisa, Bianchi Maria Luisa, Mora Stefano, Cusi Daniele, Vezzoli Giuseppe
Abstract excerpt
BACKGROUND: The aim of the present study was to investigate the interactions between the circulating concentrations of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] and the mRNA concentration of its specific nuclear receptor in human leukocytes. METHODS: We measured vitamin D receptor (VDR) mRNA extracted from leukocytes by use of TaqMan fluorescence analysis applied to the reverse transcription-PCR (RT-PCR) technique...
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