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Single-tube, nested, reverse transcriptase PCR for detection of viable Mycobacterium tuberculosis

1 May 1997

Abstract excerpt

Several problems remain before molecular biology-based techniques, such as PCR, are widely accepted for the detection of infectious agents. Among the most formidable of these problems are the inability of the tests to distinguish between viable and nonviable organisms. We approached this problem by using the fact that bacterial mRNA has an extremely short half-life, averaging only a few minutes. We reasoned that...

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PMID: 9114400
DOI: 10.1128/jcm.35.5.1161-1165.1997

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