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Detection and identification of Mycobacterium tuberculosis by DNA amplification

1 Sept 1990

Abstract excerpt

The polymerase chain reaction (PCR) was used to identify mycobacterial DNA sequences in uncultured clinical specimens. Two oligonucleotide primers derived from the sequence of a gene that codes for the 65-kilodalton antigen of Mycobacterium tuberculosis amplified DNA from all 11 species of mycobacteria tested. Amplified DNAs of nontuberculosis mycobacteria were found to be approximately 20 to 40 bases shorter...

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Identifiers

PMID: 2121786
DOI: 10.1128/jcm.28.9.1877-1880.1990

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