Article
Quantification of colony-forming units for M. tuberculosis complex using gyrB-based real-time PCR assay.
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease - 1 Jul 2016
Yin X-M, Wu L-J, Zheng L, Mao X-R, Zhang S-M, Wang Q
Abstract excerpt
BACKGROUND: It is inappropriate to select insertion sequence (IS) 6110 as the amplification target for the quantitative analysis of Mycobacterium tuberculosis complex (MTC). OBJECTIVE: To develop a novel Taqman real-time polymerase chain reaction (RT-PCR) for the quantitative analysis of MTC by amplifying a single-copy PCR target. DESIGN: Analytic sensitivity and specificity, repeatability and reproducibility,...
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