Article
Squamous cell carcinomas of the head and neck cultured in floating collagen gels: 1. The maintenance of stromal and epithelial elements in vitro without fibroblast overgrowth.
Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery - 1 Feb 1997
Burford-Mason A P, Irish J C, MacKay A J, Gullane P J, Bassett R, Dardick I
Abstract excerpt
The study of the molecular biology of head and neck squamous cell carcinomas has been heavily reliant on the analysis of cell lines. This is largely because the maintenance of primary cell cultures is difficult. However, being monoclonal, cell lines are not representative of the primary tumor because of the loss of tumor cell heterogeneity. We report a technique for primary culture of squamous cell carcinomas...
Topics
- Antibodies, Monoclonal
- Carcinoma, Squamous Cell
- Cell Survival
- Coculture Techniques
- Collagen
- Culture Techniques
- Epithelial Cells
- Fibroblasts
- Humans
- Immunohistochemistry
- Infant, Newborn
- Phenotype