Article
Isolation and culture of squamous cell carcinoma lines.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Purdie Karin J, Pourreyron Celine, South Andrew P
Abstract excerpt
Cutaneous squamous cell carcinoma (SCC) keratinocytes readily grow, expand in culture, and continuously passage, suggesting either spontaneous immortalisation at the early stage of culture or inherent proliferative capacity. One feature of SCC keratinocytes is genomic DNA rearrangement and single-nucleotide polymorphism studies of fresh frozen primary tumour, early and late passage SCC keratinocytes suggest that...
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