Characterization of human breast cancer epithelial cells (HBCEC) derived from long term cultured biopsies

doi:10.1186/1756-9966-28-127

Article

Characterization of human breast cancer epithelial cells (HBCEC) derived from long term cultured biopsies

14 Sept 2009

Abstract excerpt

INTRODUCTION: For a more individualized therapeutic approach we explored a protease-free method to culture primary cells from breast cancer biopsies. METHODS AND RESULTS: Tumor tissue from breast cancer patients after surgery was cultured ex vivo without enzymatic digestion for more than one year and revealed the continuous outgrowth of adherent and proliferating primary cell populations. Immunofluorescence...

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