Cryopreserved GABAergic neurons in cultures of rat cerebral cortex and mesencephalon: a comparative morphometric study with anti-GABA antibodies.

Abstract excerpt

Blocks of embryonic rat cerebral cortex and mesencephalon were cryopreserved and stored for up to 1 year in liquid nitrogen at -196 degrees C with 7.5% dimethylsulfoxide (DMSO) as cryoprotectant. After thawing, these tissues were only mechanically dissociated and the cells were cultured for 2-7 weeks before immunocytochemical staining with anti-GABA (gamma-aminobutyric acid) antibodies. The freeze-stored...

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