Article
Analysis of glycosylation sites on gp91phox, the flavocytochrome of the NADPH oxidase, by site-directed mutagenesis and translation in vitro.
The Biochemical journal - 1 Feb 1997
Wallach T M, Segal A W
Abstract excerpt
Flavocytochrome b558 of the NADPH oxidase which generates superoxide in phagocytic cells, is a alpha1 beta1 heterodimer of gp91phox and p22phox, which together form a membrane-spanning electron-transport chain that transfers electrons from NADPH in the cytosol to oxygen. The C-terminal portion of...
Topics
- Cytochrome b Group
- DNA Primers
- DNA, Complementary
- Dimerization
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Expression
- Glycosylation
- Granulomatous Disease, Chronic
- Humans
- Membrane Glycoproteins
- Membrane Proteins
- Mutagenesis, Site-Directed
- Mutation
- NADPH Oxidase 2
- NADPH Oxidases
- Phagocytes
- Protein Biosynthesis