Article
Technical note: use of a PCR-single strand conformation polymorphism (PCR-SSCP) for detection of a point mutation in the swine ryanodine receptor (RYR1) gene.
Journal of animal science - 1 Dec 1996
Nakajima E, Matsumoto T, Yamada R, Kawakami K, Takeda K, Ohnishi A, Komatsu M
Abstract excerpt
We used the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis for screening the point mutation (C1843 to T) in the porcine ryanodine receptor (RYR1) gene. The PCR products (659 bp) were heat-denatured and separated by polyacrylamide gel electrophoresis. On silv...
Topics
- Animals
- Base Sequence
- Calcium Channels
- DNA
- Electrophoresis, Polyacrylamide Gel
- Female
- Genes
- Genotype
- Male
- Muscle Proteins
- Point Mutation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Polymorphism, Single-Stranded Conformational