Article
Cloning of stress tolerance gene in Torulaspora delbrueckii No. 3110.
Bioscience, biotechnology, and biochemistry - 1 Oct 1996
Nakata K, Okamura K
Abstract excerpt
A cryptic plasmid (pSRY1) was found in Torulaspora delbrueckii No. 3110. A plasmid vector (pSRY21) was constructed by ligating it to pPR1-RIM-C ORF with a cycloheximide resistance gene (RIM-C). The transformation efficiency of the strain No. 3110 with pSRY21 by the protoplast-PEG method was increased by 3000-fold by treatment of the protoplasts with polylysine [poly(epsilon-L-lysine)]. Previously we have reported...
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