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Article

Recombinant vesicular stomatitis viruses from DNA.

9 May 1995

Abstract excerpt

We assembled a DNA clone containing the 11,161-nt sequence of the prototype rhabdovirus, vesicular stomatitis virus (VSV), such that it could be transcribed by the bacteriophage T7 RNA polymerase to yield a full-length positive-strand RNA complementary to the VSV genome. Expression of this RNA in cells also expressing the VSV nucleocapsid protein and the two VSV polymerase subunits resulted in production of VSV...

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PMID: 7753828
DOI: 10.1073/pnas.92.10.4477

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