Purification and characterization of the DNA‐binding protein DnrI, a transcriptional factor of daunorubicin biosynthesis in Streptomyces peucetius

Abstract excerpt

The DnrI protein, essential for the biosynthesis of daunorubicin in Streptomyces peucetius, was purified almost to homogeneity from dnrI expression strains of Escherichia coll and S. peucetius through several steps of chromatography. The proteins purified from both organisms had identical chromatographic and electrophoretic behaviour. Purified Histagged or native DnrI was used to conduct DNA-binding assays by gel...

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