Article
Amino acid substitutions in membrane-spanning domains of Hol1, a member of the major facilitator superfamily of transporters, confer nonselective cation uptake in Saccharomyces cerevisiae.
Journal of bacteriology - 1 Dec 1996
Wright M B, Howell E A, Gaber R F
Abstract excerpt
Selection for the ability of Saccharomyces cerevisiae cells to take up histidinol, the biosynthetic precursor to histidine, results in dominant mutations at HOL1. The DNA sequence of HOL1 was determined, and it predicts a 65-kDa protein related to the major facilitator family (drug resistance sub...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Carrier Proteins
- Cations, Divalent
- DNA, Fungal
- Gene Deletion
- Histidinol
- Membrane Proteins
- Molecular Sequence Data
- Open Reading Frames
- Potassium
- Protein Biosynthesis
- Saccharomyces cerevisiae